high performance liquid chromatography

High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry. It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixture by using a variety of chemical interactions between the substance being analyzed (analyte) and the chromatography column.
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HPLC. An efficient form of column chromatography that pumps a liquid solution of the sample at very high pressure through a column packed with a stationary phase made of very tiny particles. The high pressure pumps required make HPLC an expensive technique.