RT-PCR
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Reverse transcription polymerase chain reaction
In
molecular biology
, reverse transcription polymerase chain reaction (RT-PCR) is a
laboratory technique
for amplifying a defined piece of a
ribonucleic acid
(RNA) molecule. The RNA strand is first
reverse transcribed
into its
DNA
complement or
complementary DNA
, followed by amplification of the resulting DNA using
polymerase chain reaction
. This can either be a 1 or 2 step process.
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