PCR

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PCR (polymerase chain reaction)
laboratory technique used to replicate segments of DNA by repeatedly splitting the DNA strands and duplicating them with a DNA polymerase enzyme (Molecular Biology)


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Phosphocreatine
Phosphocreatine, also known as creatine phosphate or Pcr, is a phosphorylated creatine molecule that is an important energy store in skeletal muscle. It is used to anaerobically generate ATP from ADP, forming creatine for the 2 to 7 seconds following an intense effort. It does that by donating a phosphate group and this reaction is catalyzed by creatine kinase (presence of creatine kinase in plasma is indicative of tissue damage and is used in the diagnosis of myocardial infarction). This reaction is reversible and it therefore acts as a spatial and temporal buffer of ATP concentration. In other words, Phosphocreatine is part of a coupled reaction; the energy given off from one reaction is used to regenerate the other compound - be it ATP or PC. Phosphocreatine plays a particularly important role in tissues that have high, fluctuating energy demands such as muscle and brain.
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Polymerase chain reaction
The polymerase chain reaction (PCR) is a biochemistry and molecular biology technique for exponentially amplifying a fragment of DNA, via enzymatic replication, without using a living organism (such as E. coli or yeast). PCR can be used for amplification of a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece. As PCR is an in vitro technique, it can be performed without restrictions on the form of DNA, and it can be extensively modified to perform a wide array of genetic manipulations.
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iMedixDownload this dictionary
PCR
PCR polymerase chain reaction. PCR abbr. polymerase chain reaction Polymerase chain reaction A test performed to evaluate false-negative results to [more]PCR - Community and Resources

Dictionary of MedicineDownload this dictionary
PCR
Stands for Polymerase Chain Reaction, a key technique in molecular genetics that permits the analysis of any short sequence of RNA or DNA without having to clone it.

NCI Dictionary of Cancer Terms Download this dictionary
PCR
Polymerase chain reaction. A laboratory method used to make many copies of a specific DNA sequence.

A Service of the National Cancer Institute.

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